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KMID : 0895820100200020051
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Journal of Oriental Rehabilitation Medicine
2010 Volume.20 No. 2 p.51 ~ p.61
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Effects of Curcumin Analogues and Metabolite on Oxidative Stress-induced Cytotoxicity in HepG2 Cells
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Kim Ki-Byoung
Yeom Seung-Ryong
Kwon Young-Dal
Lee Su-Kyung
Song Yuug-Sun
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Abstract
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Objectives : The purpose of this study was to investigate antioxidant effects of curcumin from Curcumae Longae Radix.
Methods : Using HepG2 liver-like cells, the antioxidant effects of curcumin, one of main components from Curcumae Longae Radix, and its analogues have been evaluated by measuring their effects on cytotoxicity induced by H2O2.
Results : The pre-incubation for 6 hours with curcumin, bis-demethoxycurcumin, or dimethoxycurcumin protected HepG2 cells from H2O2-induced toxicity in a dose-dependent manner. However, tetrahydrocurcumin, one of curcumin metabolites, did not protect HepG2 cells from H2O2-induced toxicity. Interestingly, curcumin, bis-demethoxycurcumin, and dimethoxycurcumin were increased in the protein levels of heme oxygenase-1(HO-1) at concentrations that were also effective in cellular protection. In contrast, tetrahydrocurcumin did not induce HO-1 expression. Tin protoporphyrin-IX, an inhibitor of HO-1 activity, significantly abolished cytoprotection afforded by curcumin, bis-demethoxycurcumin and dimethoxycurcumin.
Conclusions : These results demonstrate that curcumin, bis-demethoxycurcumin, and dimethoxycurcumin with two conjugated doble bonds on their structures may reduce H2O2-induced oxidative stress through HO-1 expression. HO-1 induction may be one of antioxidant pathways by which curcumin protects from oxidative stress-induced cytotoxicity.
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KEYWORD
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Curcumae Longae Radix, Curcumin, Bis-demethoxycurcumin, Dimethoxycurcumin, Tetrahydrocurcumin, Oxidative stress
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